Non-replicative antibiotic resistance-free DNA vaccine encoding S and N proteins induces full protection in mice against SARS-CoV-2

Non-replicative antibiotic resistance-free DNA vaccine encoding S and N proteins induces full protection in mice against SARS-CoV-2

Blog Article

SARS-CoV-2 vaccines currently in use have contributed to controlling the COVID-19 pandemic.Notwithstanding, the high mutation rate, fundamentally in the spike Ship Model Kit glycoprotein (S), is causing the emergence of new variants.Solely utilizing this antigen is a drawback that may reduce the efficacy of these vaccines.Herein we present a DNA vaccine candidate that contains the genes encoding the S and the nucleocapsid (N) proteins implemented into the non-replicative mammalian expression plasmid vector, pPAL.

This plasmid lacks antibiotic resistance genes and contains an alternative selectable marker for production.The S gene sequence was modified to avoid furin cleavage (Sfs).Potent humoral and cellular immune responses were observed in C57BL/6J mice vaccinated with pPAL-Sfs + pPAL-N following a prime/boost regimen by the intramuscular route applying toys in vivo electroporation.The immunogen fully protected K18-hACE2 mice against a lethal dose (105 PFU) of SARS-CoV-2.

Viral replication was completely controlled in the lungs, brain, and heart of vaccinated mice.Therefore, pPAL-Sfs + pPAL-N is a promising DNA vaccine candidate for protection from COVID-19.